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            ţѪ嵰׌Ӳ֬֬ҴΌĤYӰ-Ŀ-ˮؼw-Ĥ|Ĥw-w-ĤY˾-ϺAϲĤY޹˾
            Q Ŀ „ӑB ĤĽB PAϲ “ϵʽ EN
            > „ӑB > ˾ӑB

            ţѪ嵰׌Ӳ֬֬ҴΌĤYӰ

            lrg:2019919 c:1423

            ښ/Һоg໥ԼӑB^˽swϵY͹ܵҪ;[1].֬Ҵ (PE) Ĥк^S֬, ڸ߶ȻSĤк., ֬ҴڴXк_30%~70%, ڸЃHռ֬5%[2,3].īI[4, 5], PEĎp HֵӰ.2.3<p H<8r, PE, в朵PE֬pĤ;p H>8r, PE^F, 늺֮goųʹPE^FЧMe.ڸp Hֵh, PE֬pĤǷ.Ӳ֬֬Ҵ (DSPE) ֬|wdˎwϵ, ˎﷀЧ, ӽYD1ʾ.֬|wͨ^o}עMѪҺ, YѸٰlۼB©|w׃.֬|wĽMɳɷ֛Q֬|wMѪҺО׃., ֬|w΢򲻺đ̴r, ֬|w֬tDƽoѪҺ (Ѫ嵰) [6].

            Fig.1 Molecular structure of DSPE

            Fig.1 Molecular structure of DSPE   dԭD

            Ѫ嵰Ѫ{Sĵ|[7,8], Ѫ嵰 (HSA) 585ᚈMɵĆ朵|, 35װM17I1ɵİװ[9].HSAwѪҺе﹦Ҫdw, \ݔظN֬xĴxUˎ, ͬr߀𵽾S֝B͸_p H[10].HSAж๦ܵD\, ڶNԴԺԴ|\ݔͳeҪ[11,12].ţѪ嵰 (BSA) ţѪеҪ|, 582ᚈM, 35װM17I1λ朵34λ[13].BSAԴSɱ, ھwYԼcHSAƵĽY|, ʹBSAVtˎI[14~16].Ѫ嵰wdw, ˎdwú, xDSPE֬ӿܱѪ嵰D\., оBSAcDSPEg໥ÙCƾҪWx.

            Langmuir-BlodgettgYԭ@΢R (AFM) [17~20], о˲ͬp Hֵlš/ҺBSADSPEΌĤYӰ.ßWԼW˷g໥, ôֱƂ˲ͬlµDSPEΌĤ, ԭ@΢R^y֬ĤΑBY.

            1 򞲿

            1.1 ԇcx

            Ӳ֬֬Ҵ (DSPE) ţѪ嵰 (BSA) ُSigmaԇ˾;ȷҴԇ, ُ򻯹˾;ˮȥxˮxӽQsˮ.

            KSV-MinitroughLangmuirĤƽx (mKSV˾) , :323 mm75 mm, xԎKWilhelmyƬ鉺rOyηĤı扺, yȞ0.01 m N/m;WET-SPM-9500-J3ԭ@΢R (AFM, ձu˾) .

            1.2 DSPEΌĤı扺-ƽe (-A) ͉sѭhy

            } (PBS) _Һ, BSAĵcs4.8, ͨ^HClNa OHp Hֵ{3, 5, 79.BSAܽsˮ, ƝȞ3 mg/m LҺ.BSAKȞ5, 10, 20, 3050 nmol/L, ˝, ]֬ӕr, BSA扺Ӱ푿ԺԲӋ.DSPEܽV (ȷ) V (״) =31܄, ƝȞ0.2 mg/m LҺ.΢Mһwe֬ҺμӵPBS, ʹԄչ, o15 min, ҺwЙC܄ȫ]l, 10 mm/minٶȉĤ.-AӋCԄӫ@.

            s-≺sѭh:O㶨ĿĤ, ΌĤ^Bms͔Uѭh, sѭhӋCԄӫ@.ßoˮҴϴ, ȥxˮصϴ.ÿM؏3.ضȿ (181) .

            1.3 扺-rg (-t) ƽe-rg (A-t) y

            ΢MһweDSPE֬ҺμӵPBSҺ, ʹԄչ, o15 min, ҺwЙC܄ȫ]l, 10 mm/minٶȉĤ.Ĥ扺_20 m N/mr, ֹͣs, ΢MһweBSAעPBS (BSAKȷքe5, 10, 20, 3050 nmol/L) .̶e, ӛĤ扺Srg׃r.ƽe-rg (A-t) Ĝy^c-tą^eڮĤ扺_20 m N/mr, ̶Ĥ, ӛƽeSrg׃r.ضȿ (181) .

            1.4 AFMzy

            ̶Ĥ, ôֱĤ1 mm/minٶȌDSPE֬ĤΌĤDƵ½xĸƬ, Ҝ·2 h, , AFM^yƷıò.΢ұ۵ďԳ0.06 N/m, lʞ1.0 Hz, ЈDƬɼc512512.^̾ҜM.

            2 YcӑՓ

            2.1 -AȜ

            вͬȵBSADSPEΌĤõĦ-AȜؾD2ʾ.Ҋ, ڲͬp Hֵ, DSPEΌĤı扺SƽeĜpСm, s54 m N/m.p H=3, 57r, ʼes0.65 nm2;p H=9r, ʼes0.72 nm2.p H=9r, DSPE^FʬF, oų⌧DSPE^FęMe[5].p H=7r, мBSAӺ, Ĥ<30 m N/mr, ƽeӵķƄ, fеױ;Ĥ>30 m N/mr, ƽepСķƄ.@ڵĤ, ֬ӵˮβ朲뵽ţѪ嵰׵ˮ΢^, SsM, ֵ׷ӱDM, ͬr߲DSPE֬.

            Fig.2 Surface pressure () -mean molecular area (A) isotherms of DSPE monolayers

            Fig.2 Surface pressure () -mean molecular area (A) isotherms of DSPE monolayers   dԭD

            (A) p H=3; (B) p H=5; (C) p H=7; (D) p H=9.

            MһBSAĤs׃̶, ӋĤքe2040 m N/mr, DSPE֬Ĥƽe׃A (A=A-A0) , YD3ʾ.Ҋ, p H=7r, AСr, f˕rBSAcDSPEg໥, @BSAԓp HֵŽؓ, ʬFoܵĽY, ˮ¶.p H=9r, Ap H=7r, @p H=9r, PE^FcBSA, BSAʬFɢĽY, ˮ¶, ˕rÞˮúoYϵķʽ.p H=3r, ĤƽeӵķƄ, fBSAʬFLĽY, ˮ׺ȫ¶r, cDSPEg໥.Ĥ20 m N/mr, p H=5rAֵ, Ĥ40 m N/m, p H=3rAֵ, @fBSAcDSPE֮g໥p HֵԼĤӰ푾^.p H=3, 57r, DSPE, BSAcDSPEgˮ.Souza[21]@ښ/Һ控xӵĶؙ֬Ҵ (N, N-Dimethyl-PE) , ͨ^Ƕ뵽BSAˮ΢^cBSAl໥, @cĽY.ɦ-A߀ɰlF, p H=7r, SBSAȵ, ҺBUcҺB۹ƽ_^, fBSAΌĤB׃.BSAȞ3050 nmol/Lr, ֬ĤĦ-A׺غ, ׷֬ĤҪ˷܄[22], ÿ_ĵ׷Ӷ֬Ĥ., ͬh, BSADSPE֬ΌĤ1څ͠Bֵ.

            Fig.3 Mean molecular area (A) -BSA concentration[c (BSA) ]isotherms of DSPE monolayers

            Fig.3 Mean molecular area (A) -BSA concentration[c (BSA) ]isotherms of DSPE monolayers   dԭD

            (A) 20 m N/m; (B) 40 m N/m.

            2.2 -t

            ڳʼĤ20 m N/m, BSAвͬȕr, DSPEΌĤĦ-tD4ʾ.Ҋ, ĤȴһpС^, sֹͣ֬ڽϴżBSAāДUɢ^.BSAȺp Hֵĸ׃ʹĤƽĤl׃.SBSAǶDSPE֬ΌĤ, ƽ≺׃1ʾ.

            Fig.4 Surface pressure () -time (t) curves of pure DSPE monolayers and DSPE containing BSA (20, 50 nmol/L) monolayers on PBS subphase

            Fig.4 Surface pressure () -time (t) curves of pure DSPE monolayers and DSPE containing BSA (20, 50 nmol/L) monolayers on PBS subphase   dԭD

            (A) p H=3; (B) p H=5; (C) p H=7; (D) p H=9.

            Table 1 Parameter of-t curves for pure DSPE monolayers and DSPE containing BSA monolayers at different subphase p H values*     

            Table 1 Parameter of-t curves for pure DSPE monolayers and DSPE containing BSA monolayers at different subphase p H values*

            *c (BSA) =0 (0) , 20 (1) and 50 nmol/L (2) .

            ɱ1֪, p Hֵͬ, c (BSA) =50 nmol/Lr, ƽĤ׃2c (BSA) =20 nmol/LrƽĤ׃1.BSAͬ, p H=3r, Ĥ׃;p H=7r, Ĥ׃С.@fp H=3r, BSAcDSPE֮g໥, BSAӱ.Sp Hֵ, p H=7r, BSA, f˕rBSAĎԼYcʹcDSPEӵ໥^.˽Yc-AYһ.

            ˷BSAǶDSPE֬ĤĄW^, 򞿼ڲͬp Hֵl, c (BSA) =50nmol/LrĤ扺Srg׃r, YD5ʾ, ͨ^ʽ[23]D5MһCM, Y2ʾ.

             

            ʽ:abքe֬ĤijʼK扺;k^һʳ.

            Fig.5 Adsorption kinetics of BSA at different subphase p H values

            Fig.5 Adsorption kinetics of BSA at different subphase p H values   dԭD

            c (BSA) =50 nmol/L.Curved lines are the fitting curves using eq. (1) .Zero time is adjusted to the time when the rising of pressure starts.

            ɈD5֪, Y͔MǺ^, һһABSAΌĤеĄW^.ı2֪, ځչDSPEΌĤ, һʳkֵkp H=5<kp H=9<kp H=7<kp H=3, fDSPEcBSA֮g໥ÏӰDSPEBSAr.Pedraz[24], BSAځеĔUɢٶSp HֵӶ.p H=3r, mȻBSAځДUɢٶ, BSAcDSPE֮gҵˮ, ʹ^BSA, kֵ.p H=5r, kֵС, @ڴ˕rBSAӳһNoĽY, BSAӔUɢٶ^, BSAcDSPE֮g໥Ïȵp H=3rď., p H=5rDSPEBSA^, KBSAӱp H=79r.p H=9r, mȻBSAĔUɢٶȱp H=7r, ڴ˕rDSPE^F, ֬ΌĤ^, ӵ^, ͬrBSAٔUɢcDSPEl໥, ˽扺׃, kֵ^С.p H=7r, ֬ΌĤ^, ֬ӵÌBSAδ_r, 扺ѸٜpС;BSA, Ĥ扺Ѹ, kp H=9<kp H=7.lF, BSAĝȾ50 nmol/Lr, DSPE/BSAΌĤƽĤp HֵӰ^, fBSADSPEΌĤֵ, ԓֵĴС܁p HֵӰ.@ځp HֵӰBSAĽY͎ԼDSPEĎ, ĶDSPEBSAa.

            Table 2 Fitting parameters using eq. (1) of adsorption kinetics of BSA at different subphase p H values*     

            Table 2 Fitting parameters using eq. (1) of adsorption kinetics of BSA at different subphase p H values*

            *The values a and b are the initial and final surface pressure of the monolayer and k is the apparent first-order rate constant.R is the residual correlation coefficient.c (BSA) =50 nmol/L.

            2.3 ĤԷ

            BSAӌDSPE֬ĤYԵӰ푿ͨ^A/A0 (A0t=0rƽe, Atr̵ƽe) Srg (t) ׃rõ[25].͹, epʧڽĤıɵ;鰼, tHԷM끆·epС[24].ڌʹ֬Ĥ߻ĤĤ35 m N/m, ^ƽeSrg׃r.ɈD6Ҋ, 鰼, fɷepСԭǃHԷ끆, DSPEĤķp HֵBSAӰ.p H=3, 59r, ֬ĤcBSArغ;p H=7r, BSA, A/A0ֵҪȼ֬Ĥĵ.@p H=7r, BSAcDSPE֮g໥^, BSAuM뵽, y֬M끆, ƽepС, ĶĤķ.

            BSAӌDSPE֬ĤYԵӰҲͨ^Bmĉs-≺sѭh_[21,26].ڲͬp Hֵ, DSPE/BSAΌĤĤ35 m N/mr, s-≺sѭhD7ʾ.xȡDSPEΌĤ錦սM, BSAĝȷքe2050 nmol/L.Ҋ, p Hֵ, DSPEΌĤ35 m N/mrĉsͽ≺sغ, СĜF, @֬ڽ.p H=3, BSA, sc≺sքeغ^, fBSAcDSPE֮g^໥, ʹBSAǶDSPE֬Ĥ, ĶĤķ.p H=5r, sͽ≺sܺܺõغ, fBSAǶDSPE֬Ĥķ.p H=79r, DSPE֬ĤF΢;BSA, F׃@.@DSPE/BSAYډs^бDM, ԱچΌĤ·, ڷg^, Ĥ^Сr, ֱַ.s-≺sѭhĜFp H=7r@, BSAȞ50 nmol/Lr, DSPE/BSAΌĤĉsͽ≺sغҪp H=9r (Ĥ15 m N/mr, 3ѭh^Љs-≺s^ƽe׃քep H=7r, A1=0.1766 nm2, A2=0.1288 nm2, A3=0.1040 nm2;p H=9r, A1=0.1706 nm2, A2=0.1274 nm2, A3=0.1030 nm2) .˕r, s-≺sѭhеĜجFډs^BSAcDSPE໥^, DDSPEΌĤBSADSPEΌĤķԽ.

            Fig.6 Stability of pure DSPE monolayers and DSPE containing BSA (20 nmol/L) monolayers at different subphase p H values

            Fig.6 Stability of pure DSPE monolayers and DSPE containing BSA (20 nmol/L) monolayers at different subphase p H values  dԭD

            (A) p H=3; (B) p H=5; (C) p H=7; (D) p H=9.

            Fig.7 Compression-expansion cycles of pure DSPE and DSPE containing BSA at different subphase p H values

            Fig.7 Compression-expansion cycles of pure DSPE and DSPE containing BSA at different subphase p H values   dԭD

            (A) p H=3; (B) p H=5; (C) p H=7; (D) p H=9.

            Fig.8 Model of the process of successive compression-decompression cycles

            Fig.8 Model of the process of successive compression-decompression cycles   dԭD

            ڲͬp Hֵ, ֬Ĥĉsc≺s^̿ģ͸ֱ^ӑB^ (D8) ., BSAcDSPE֮g໥ôʹBSAӱ, γDSPE/BSAY[D8 (A) ], ԓYډs^бDM, ԱچΌĤ·[D8 (B) ], չ_, Ĥ扺׃Сr, BSAֱ[D8 (C) ].

            2.4 sԷ

            оͬBSADSPEΌĤόĤsԵӰ, ӋˆΌĤďģ (Cs-1) , Cs-1DZΌĤı扺sԼ׃ОҪ, ɦ-AӋ[3,27,28]:

             

            ʽ, Aͦзքeƽeͱ扺.

            ģc扺֮gPϵD9ʾ.Ҋ, p H=3r, SBSA, Cs-1ֵ220 Mn/m͵132 m N/m, BSAǶ뵽DSPE֬Ĥ, ֬Ĥĉs.p H=5r, ֬ĤCs-1ֵ212 m N/m-1, BSAȞ10 nmol/Lr, SBSA, Cs-1ֵpС, fΌĤBSAˆΌĤĉs, BSAȞ10nmol/Lr, Cs-1ֵ, fԓ^BSADM, aĿλĔ^, ĶsԱr.p H=79r, ʬFͬҎ.īI[29], Cs-1OСֵ̎λÌ֬ΌĤl׃׃c.ɈD9֪, p H=5, 79, Ĥ23~26 m N/mr, FOСֵ, ҘOСֵSBSAȵĤpСķƄ, @fSBSAǶ, ֬ĤFҺBUൽҺBUcҺB۹׃, ׃cĤSBSAȵӶpС.p Hֵ, дBSAr, BSAǶ뵽DSPE֬Ĥ, ֬Ĥĉs.֬Ĥ׃Оp HֵӰҲ^.

            Fig.9 Compressibility coefficient (Cs-1) -surface pressure () curves of DSPE monolayers

            Fig.9 Compressibility coefficient (Cs-1) -surface pressure () curves of DSPE monolayers   dԭD

            (A) p H=3; (B) p H=5; (C) p H=7; (D) p H=9.

            2.5 AFM

            AFMڼ{׷õƷıòYD, Ķṩg໥úͽMֵĻܵϢ[30~33].^, ΌĤDĸƬ (Ĥ̶2040 m N/m) MAFM^y.p H=3, 5, 79r, ͬBSA (Ȟ020 nmol/L) cDSPE֬ΌĤ໥õAFMDD10ʾ.Ҋ, Ĥ́p HֵӰ푼DSPE֬ĤòY.ͬp Hֵ, ĤԽ, ֬ĤYԽo[D10 (A) c10 (B) , D10 (E) c10 (F) , D10 (I) c10 (J) , D10 (M) 10 (N) ].=20 m N/mr, Sp Hֵ, DSPE֬Ĥķ֧Yuγ^ƽ_Y[D10 (A) , (E) , (I) (M) ].дBSAӕr, BSAǶDSPE֬Ĥ֬ĤYкܴӰ.p H=3r, ͬĤ¼DSPE֬ĤcDSPE/BSAĤlF, BSAǶʹĤķ֧YpС, γ^ƽ_Y, AFMDаlFc[D10 (C) (D) ], BSAǶDSPEĤ.p H=5r, ځмBSA, AFMDͬӰlFBSA, ˕rBSAӵĔĿ@p H=3r[D10 (G) (H) ], BSAǶʹ֬Ĥ^ƽ_Yʧ[D10 (F) (H) ].p H=7r, AFMDҊ, BSAĔĿ[ (D10 (K) (I) ], f˕rBSAcDSPE֮g໥^΢, ٵBSAӱ., ڵĤ, BSAļʹĤY׃ɸoƽ_Y;ڸĤ, ԭ^ƽ_YsƉ, @BSAcDSPEg^΢, ډs^, BSAӱD沢y֬M끆ɵ, @c-Aͦ-tYһ.p H=9r, ֬ĤĽY׃rcp H=7r, ֻp H=9r^쵽^BSA[ (D10 (O) (P) ], ĤĤY׃̶^, @fBSAӱDM, ĶҲCp H=9rBSAcDSPEg໥ñp H=7r.

            Fig.10 AFM images of pure DSPE and mixed DSPE/BSA monolayers at differentand p H values

            Fig.10 AFM images of pure DSPE and mixed DSPE/BSA monolayers at differentand p H values   dԭD

            (A) DSPE, p H=3, =20 m N/m; (B) DSPE, p H=3, =40 m N/m; (C) DSPE-BSA, p H=3, =20 m N/m; (D) DSPE-BSA, p H=3, =40 m N/m; (E) DSPE, p H=5, =20 m N/m; (F) DSPE, p H=5, =40 m N/m; (G) DSPE-BSA, p H=5, =20 m N/m; (H) DSPE-BSA, p H=5, =40 m N/m; (I) DSPE, p H=7, =20 m N/m; (J) DSPE, p H=7, =40 m N/m; (K) DSPE-BSA, p H=7, =20 m N/m; (L) DSPE-BSA, p H=7, =40 m N/m; (M) DSPE, p H=9, =20 m N/m; (N) DSPE, p H=9, =40 m N/m; (O) DSPE-BSA, p H=9, =20 m N/m; (P) DSPE-BSA, p H=9, =40 m N/m.c (BSA) =20 nmol/L.Scanning range:15m15m.

            3 YՓ

            о˲ͬp Hֵh, вͬȵţѪ嵰 (BSA) Ӳ֬֬Ҵ (DSPE) ΌĤYӰ.Y, BSAcDSPE֮g໥܁p HֵĤӰ푾^.p H=3r, 2N֮g໥;p H=7r, 2N֮g໥.ԭǮp H=3r, BSAˮ׺ȫ¶;p H=7r, BSAHˮ¶.ͨ^Ȍ򞔵֪, p H=3, 57rBSAcDSPEgˮ;p H=9r, gÞˮúoýYϵķʽ.BSADSPE֬ĤЕDSPE֬Ĥĉs, ҕ׃֬Ĥ׃О.p H=7r, BSAļDSPE֬Ĥķ.WY, DSPEcBSAĽYһ^, BSAچΌĤֵ, ԓֵĴС܁p HֵӰ.ΑB^yY, BSAcDSPE֮g໥ԼĤ扺֬ΌĤYl׃.

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